The β-d-xylosidase of Trichoderma reesei is a multifunctional β-d-xylan xylohydrolase
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چکیده
منابع مشابه
A novel xylan degrading β-D-xylosidase: purification and biochemical characterization.
Aspergillus ochraceus, a thermotolerant fungus isolated in Brazil from decomposing materials, produced an extracellular β-xylosidase that was purified using DEAE-cellulose ion exchange chromatography, Sephadex G-100 and Biogel P-60 gel filtration. β-xylosidase is a glycoprotein (39 % carbohydrate content) and has a molecular mass of 137 kDa by SDS-PAGE, with optimal temperature and pH at 70 °C ...
متن کاملStructure–Function Relationships of a Catalytically Efficient β-D-Xylosidase
β-D-Xylosidase from Selenomonas ruminantium is revealed as the best catalyst known (kcat, kcat/Km) for promoting hydrolysis of 1,4-β-D-xylooligosaccharides. 1H nuclear magnetic resonance experiments indicate the family 43 glycoside hydrolase acts through an inversion mechanism on substrates 4-nitrophenylβ-D-xylopyranoside (4NPX) and 1,4-β-D-xylobiose (X2). Progress curves of 4-nitrophenyl-β-D-x...
متن کاملCloning, expression and characterization of a β-d-xylosidase from Lactobacillus rossiae DSM 15814T
BACKGROUND Among the oligosaccharides that may positively affect the gut microbiota, xylo-oligosaccharides (XOS) and arabinoxylan oligosaccharides (AXOS) possess promising functional properties. Ingestion of XOS has been reported to contribute to anti-oxidant, anti-bacterial, immune-modulatory and anti-diabetic activities. Because of the structural complexity and chemical heterogeneity, complet...
متن کاملβ-d-Altrose
The mol-ecule of the title compound, C(6)H(12)O(6), [systematic name: (2R,3S,4R,5R,6R)-6-(hydroxy-meth-yl)oxane-2,3,4,5-tetrol] adopts a (4)C(1) chair conformation with the anomeric hydroxyl group in the equatorial position. All hydroxyl groups act as donors and acceptors in hydrogen bonding and the mol-ecule is involved in ten inter-molecular O-H⋯O inter-actions [O⋯O = 2.672 (5)-2.776 (4) Å] w...
متن کاملβ-d-Gulose
The title compound, C6H12O6, a C-3 position epimer of d-galactose, crystallized from an aqueous solution, was confirmed as β-d-pyran-ose with a (4) C 1 (C1) conformation. In the crystal, O-H⋯O hydrogen bonds between the hy-droxy groups at the C-1 and C-6 positions connect mol-ecules into a tape structure with an R 3 (3)(11) ring motif running along the a-axis direction. The tapes are connected ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1997
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3210375